Molecular fundamentals of enzymology

General data

Course ID:	1000-714MPE
Erasmus code / ISCED:	13.6 The subject classification code consists of three to five digits, where the first three represent the classification of the discipline according to the Discipline code list applicable to the Socrates/Erasmus program, the fourth (usually 0) - possible further specification of discipline information, the fifth - the degree of subject determined based on the year of study for which the subject is intended. / (0512) Biochemistry The ISCED (International Standard Classification of Education) code has been designed by UNESCO.
Course title:	Molecular fundamentals of enzymology
Name in Polish:	Molekularne podstawy enzymologii
Organizational unit:	Faculty of Mathematics, Informatics, and Mechanics
Course groups:
ECTS credit allocation (and other scores):	(not available) Basic information on ECTS credits allocation principles: the annual hourly workload of the student’s work required to achieve the expected learning outcomes for a given stage is 1500-1800h, corresponding to 60 ECTS; the student’s weekly hourly workload is 45 h; 1 ECTS point corresponds to 25-30 hours of student work needed to achieve the assumed learning outcomes; weekly student workload necessary to achieve the assumed learning outcomes allows to obtain 1.5 ECTS; work required to pass the course, which has been assigned 3 ECTS, constitutes 10% of the semester student load. view allocation of credits
Language:	Polish
Type of course:	obligatory courses
Short description:	During the lecture, students will learn about the structure of enzymatic proteins, the mechanisms of enzymatic catalysis, and enzymatic kinetics. The classes will present techniques and methods useful in working with enzymes in vitro and in silico.
Full description:	Lecture: Course content: levels of protein structural organization, structural motifs, structural classification of proteins, forces shaping spatial structures, folding, basics of chemical thermodynamics, the structure of enzymatic proteins, mechanisms of enzymatic catalysis, types of enzymatic reaction kinetics, types of catalytic reactions and enzyme classification, membrane bioenergetics systems. Classes: 1. Biochemical and enzymatic calculations. Basics of spectrophotometry. 2. Basics of simulation of chemical and enzymatic reactions using the Gepasi computer program. 3. Basics of enzymatic reaction measurements. Determination of the activity of transaminases in the rabbit blood serum. 4. Experimental determination of the kinetic parameters Km and Vmax for L-lactate dehydrogenase. 5. Mathematical methods for the determination of kinetic parameters of enzymes. 6. Inhibition of enzymatic reactions - simulations in the Gepasi program and data analysis. 7. Mechanisms of enzymatic reactions - simulations in the Gepasi program and data analysis. 8. Application of computer programs to analyze the spatial structure of enzymes.
Bibliography:	(in Polish) C. Branden, J. Tooze. Introduction to Protein Structure 2 edition Garland Science; 2 edition, 1999 J.M Berg, J.L. Tymoczko, L. Stryer Biochemia, Rozdziały 3,4, 8-10, PWN, Warszawa 2005 A. Zgirski, R. Gondko Obliczenia Biochemiczne, PWN, Warszawa 1998 http://www.chem.qmul.ac.uk/iubmb/enzyme/ http://www.gepasi.org
Learning outcomes:	(in Polish) Wiedza: ma wiedzę na temat podstaw fizykochemicznych katalizy enzymatycznej (K_W03) Umiejętności: potrafi dokonać analizy i symulacji sieci biochemicznych na różnych poziomach przybliżeń (K_U23) potrafi modelować układy biomolekularne oraz projektować inhibitory enzymów (K_U22)
Assessment methods and assessment criteria:	(in Polish) Sprawozdania z ćwiczeń oraz egzamin pisemny obejmujący wiedzę z wykładów

This course is not currently offered.

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