Experimental Embriology of Mammals

General data

Course ID:	1400-225EMBS
Erasmus code / ISCED:	13.104 Kod klasyfikacyjny przedmiotu składa się z trzech do pięciu cyfr, przy czym trzy pierwsze oznaczają klasyfikację dziedziny wg. Listy kodów dziedzin obowiązującej w programie Socrates/Erasmus, czwarta (dotąd na ogół 0) – ewentualne uszczegółowienie informacji o dyscyplinie, piąta – stopień zaawansowania przedmiotu ustalony na podstawie roku studiów, dla którego przedmiot jest przeznaczony. / (unknown)
Course title:	Experimental Embriology of Mammals
Name in Polish:	Embriologia eksperymentalna ssaków
Organizational unit:	Faculty of Biology
Course groups:	(in Polish) Przedmioty obieralne na studiach drugiego stopnia na kierunku bioinformatyka Elective subjects
ECTS credit allocation (and other scores):	(not available) Basic information on ECTS credits allocation principles: the annual hourly workload of the student’s work required to achieve the expected learning outcomes for a given stage is 1500-1800h, corresponding to 60 ECTS; the student’s weekly hourly workload is 45 h; 1 ECTS point corresponds to 25-30 hours of student work needed to achieve the assumed learning outcomes; weekly student workload necessary to achieve the assumed learning outcomes allows to obtain 1.5 ECTS; work required to pass the course, which has been assigned 3 ECTS, constitutes 10% of the semester student load. view allocation of credits
Language:	Polish
Type of course:	elective courses
Requirements:	Animal developmental biology 1400-123BRZ Developmental Biology of Animals M 1400-124BRZ-M
Prerequisites (description):	Candidates are expected to know the basics of cell biology (particularly cell cycle regulation) and the course of oogenesis, spermatogenesis, fertilisation and early mammalian development.
Mode:	Classroom
Short description:	Practicals in "Experimental embryology of mammals" are held in the first half of the first semester on Thursdays and Fridays, for 6 hrs daily.There is only one, 12-students group. Candidates are expected to be manually skilful. During the course students do by themselves simple experiments on mouse oocytes and preimplantation embryos and learn some techniques of modern mammalian embryology, such as: in vitro culture of oocytes and embryos, fertilisation in vitro, parthenogenetic activation of oocytes, detection of proteins by indirect immunofluorescence, making microscopic preparations. The practicals begin with instructions regarding the aim and the of the experimental procedure. Students are obliged to deliver protocols of three experiments. During the course each student has to present one original article related to the subject of the performed experiment.
Full description:	Practicals in "Experimental embryology of mammals" are held in the first half of the first semester on Thursdays and Fridays, for 6 hrs daily.There is only one, 12-students group. Candidates are expected to be manually skilful. During the course students do by themselves simple experiments on mouse oocytes and preimplantation embryos and learn techniques of modern mammalian embryology, such as: in vitro culture of oocytes and embryos, fertilisation in vitro, parthenogenetic activation of oocytes, detection of proteins by indirect immunofluorescence, making microscopic preparations. Practicals begin with instructions regarding the aim and the procedure of the experiment. Students work individually: disect mice, collect and handle oocytes/embryos. They are obliged to deliver protocols of three experiments. During the course each student has to present one original article related to the subject of the performed experiment. Topics: 1. Role of cAMP in meiotic maturation of mouse oocytes 2. Role of phosphodiesterases in meiotic maturation of mouse oocytes 3. Role of protein synthesis in meiosis of mouse oocytes 4. Role of CDK1 kinase in meiosis of mouse oocytes 5. Role of Ca2+ ions in fertilization-triggered activation of embryonic development 6. Role of membrane proteins in fertilization. 7. Role of spindle structure in activation of embryonic development 8. Capacity for regulative development of early mouse embryos 9. Establishment of the first embryonic cell lines
Bibliography:	Maleszewski M. (2002) Zapłodnienie i początek rozwoju ssaków. W: "Molekularne mechanizmy rozwoju zarodkowego" (red.) Krzanowska H., Sokół-Misiak W. PWN, Warszawa, str. 203-226. Wassarman P.M., Albertini D.F. (1994) The mammalian ovum. W: : "The physiology of reproduction"vol.1 (red.) Knobil E., Neill J.D. Raven Press 1994, New York, str. 79-122. Yanagimachi, R. (1994) Mammalian fertilization. W: "The physiology of reproduction"vol.1 (red) Knobil E., Neill J.D. Raven Press 1994, New York, str. 189-318. "Ćwiczenia z biologii rozwoju zwierząt" (red.) Maleszewski M., Wydawnictwa Uniwersytetu Warszawskiego 2007, Warszawa, rozdz. 7, str.98-143
Learning outcomes:	After the practicals students know the mechanisms of oocyte maturation, fertilisation, parthenogenetic activation and preimplantation embryonic development. At practical level they can recover oocytes and embryos and manipulate them under a stereoscopic microscope, perform parthenogenetic activation and in vitro insemination and make microscopic preparations. Moreover, they can interpret experimental results, write up an experimental report and present a scientific paper.
Assessment methods and assessment criteria:	Students' participation in the class is marked on the base of: 1. their dedication to work and manual skills; 2. presentation of the scientific article; 3. reports from the conducted experiments. The final exam requires: 1. knowledge from the Developmental Biology course (the part regarding development of mammals); 2. information from the scientific articles presented during the course; 3. any additional information provided by the course instructor.
Practical placement:	n/a

This course is not currently offered.

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Copyright by University of Warsaw.