Laboratory of general microbiology and bacterial genetics

General data

Course ID:	1100-2BB15
Erasmus code / ISCED:	13.4 Kod klasyfikacyjny przedmiotu składa się z trzech do pięciu cyfr, przy czym trzy pierwsze oznaczają klasyfikację dziedziny wg. Listy kodów dziedzin obowiązującej w programie Socrates/Erasmus, czwarta (dotąd na ogół 0) – ewentualne uszczegółowienie informacji o dyscyplinie, piąta – stopień zaawansowania przedmiotu ustalony na podstawie roku studiów, dla którego przedmiot jest przeznaczony. / (0511) Biology The ISCED (International Standard Classification of Education) code has been designed by UNESCO.
Course title:	Laboratory of general microbiology and bacterial genetics
Name in Polish:	Praktikum z mikrobiologii ogólnej i genetyki bakterii
Organizational unit:	Faculty of Physics
Course groups:
ECTS credit allocation (and other scores):	(not available) Basic information on ECTS credits allocation principles: the annual hourly workload of the student’s work required to achieve the expected learning outcomes for a given stage is 1500-1800h, corresponding to 60 ECTS; the student’s weekly hourly workload is 45 h; 1 ECTS point corresponds to 25-30 hours of student work needed to achieve the assumed learning outcomes; weekly student workload necessary to achieve the assumed learning outcomes allows to obtain 1.5 ECTS; work required to pass the course, which has been assigned 3 ECTS, constitutes 10% of the semester student load. view allocation of credits
Language:	Polish
Type of course:	obligatory courses
Mode:	Classroom
Short description:	The Practicum covers basic information on bacteria, relating to bacterial cell structure, forms of existence in the environment, influence of physical and chemical factors on their growth and introduces basic information on bacteria genetics, in particular the basic information about plasmids and horizontal gene transfer. The laboratory shall acknowledge the students with basic microbiologic techniques.
Full description:	The aim of the Practicum is to present the basic information on bacteria, methods of studies on bacteria and techniques of genetic engineering based on use of bacteria. Program: 1. Techniques of working in a microbiological laboratory - sterile work, microbiological media.. Isolation of pure bacterial cultures and determination of their number. 2. Morphology and structure of a bacterial cell (description based on the staining). 3. Influence of physical and chemical factors on bacterial growth. 4. Elements of bacterial genetics - plasmids, transformation, coniugation. 5. Regulation of gene expression - lactose operon of E. coli. Student's amount of work: The laboratory = 15 hours Preparation of the reports = 7.5 hours (1.5 hour per report) Unassisted preparation for the exercise = 7.5 hours Preparation for the test = 15 hours Total about 45 hours
Bibliography:	(in Polish) 1. red. J. Baj "Mikrobiologia" PWN 2018 2. P. Singleton "Bakterie w biologii,biotechnologii i medycynie”, PWN, 2000 – wybrane zagadnienia 3. W.J.H Unicki-Goldfinger „Życie bakterii” PWN 2006- wybrane zagadnienia 4. red. J. Baj, Z. Markiewicz, "Biologia molekularna bakterii", PWN, 2006 - wybrane zagadnienia
Learning outcomes:	Having learned the material covered by the classes, the student: KNOWLEDGE 1. knows the structure of a bacterial cell, structural differences between Gram-positive and Gram-negative bacteria, (K_W06) 2. knows elements of bacterial genetics: plasmids, horizontal gene transfer, (K_W06) 3. knows basic microbiologic techniques. (K_W04) ABILITIES 1. can work sterile, justify the choice of sterilisation method of solutions, media, glass and small equipment, (K_U01) 2. can isolate pure bacterial cultures and prepare bacterial cultures,(K_U01) 3. can transform bacterial cells with a plasmid vector, (K_U01) 4. can interpret simple results of experiments performed using bacteria. (K_U01, K_U06) ATTITUDES: 1. is aware of the meaning of research ethics and responsibility for reliability of the obtained results, (K_K04, K_K05) 2. understands the possibilities of using bacteria in biotechnology, medicine, environment protection and in the industry. (K_K06, K_K07)
Assessment methods and assessment criteria:	1. Submitting of the completed result cards 2. Written final test in a form of multi-choice test and tasks of short answer. The final grade is a weighted average of grades for reports (weigh 1/3) and a grade from the final test (weigh 2/3).
Practical placement:	(in Polish) Brak

This course is not currently offered.

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