Molecular biology laboratory

Passed “Introduction to Microbiology and Molecular Biology”, and course "Molecular Biology with Genetics, Part I".

Classroom

The aim of the laboratory is to teach students how to work without assistance, using methods of genetic engineering and molecular biology in a logic sequence: from design of a genetic construct coding the desired protein, via preparation of the construct, introducing it into bacteria, isolation and introductory characterisation of the protein product.

The aim of the laboratory is to acknowledge the students with chosen techniques of molecular biology.

1. Chosen methods of nucleic acids isolation and analysis.

2. Restriction enzymes - as an instrument in genetic engineering.

3. Designing of constructs based on plasmid vectors.

4. Amplification of a DNA fragment using polymerase chain reaction (PCR).

5. Cloning of a DNA fragment in E. coli on a plasmid vector.

6. Obtaining recombinant protein using a method of overexpression in bacterial cells.

7. Assessment of purity and concentration of the protein.

Student's amount of work:

The laboratory = 90 hours

Preparation of the reports = 45 hours

Unassisted preparation for the exercise = 45 hours

Preparation of a chosen scientific article = 30 hours

Total 210 hours

1. P. C. Turner, A.G. McLennan, A.D. Bates, M.R.H. White, „Biologia Molekularna" z serii "Krótkie Wykłady" - PWN, Warszawa -wybrane zagadnienia

2. T.A Brown „Genomy” PWN, Warszawa – wybrane zagadnienia

3. R. Słomki (red.) „Analiza DNA” Wydawnictwo Uniwersytetu Przyrodniczego w Poznaniu, Poznań 2008

4.T.A Brown „Gene Cloning & DNA Analysis" Blackwell Publishing -

Having learned the material covered by the classes, the student:

KNOWLEDGE

1. knows chosen methods of isolation of nucleic acids from bacterial cells, (K_W04, K_W06, K_W07)

2. knows chosen methods of analysis of nucleic acids, (K_W04, K_W06, K_W07)

3. knows a method of DNA fragment amplification using the PCR technique, (K_W04, K_W06, K_W07)

4. knows chosen methods of assessment of protein purity and concentration (K_W04, K_W06, K_W07)

5. knows chosen heterologic protein expression systems in E. coli cells. (K_W04, K_W06, K_W07)

ABILITIES

1. can isolate plasmid DNA using alkaline lysis, (K_U01, K_U04)

2. can transform bacterial cells with a plasmid vector - using a heat-shock method or via electroporation, (K_U01, K_U04)

3. can apply the PCR technique for DNA fragment amplification, (K_U01, K_U04)

4. can clone DNA fragments in E. coli on a plasmid vector,(K_U01, K_U04)

5. can obtain recombinant protein using overexpression in bacterial cells, (K_U01, K_U04)

6. can determinate protein concentration in a solution, (K_U01, K_U04)

7. can assess the purity of the obtained sample, (K_U01, K_U04)

8. can interpret the results of the performed experiments. (K_U04, K_U09)

ATTITUDES:

1. is aware of the meaning of research ethics and responsibility for reliability of the obtained results, (K_K04, K_K05)

2. understands the possibilities of using techniques of genetic engineering in biotechnology, medicine, diagnostics, criminalistics and in the industry. (K_K06, K_K07)

Submitting of the cards of every class