Analysis of biologically active compounds A - laboratory

obligatory courses

Organic Chemistry II Laboratory 1200-1CHMCHO2L3

Knowledge of the basics of organic chemistry (basic course), confirmed by a passed exams in "Organic Chemistry I & II" AND knowledge of the basics of practical organic synthesis confirmed by completing a course of "Organic Chemistry II Laboratory".

Classroom

During the laboratory, students learn practical aspects of modern analysis of organic compounds, including non-hazardous bioactive compounds. Students practice methods of isolation and purification of organic compounds and practice contemporary methods of identification and determination of structure and purity of compounds using chromatographic, spectroscopic and chemical analysis techniques.

The laboratory covers the basics of analysis of organic compounds, including bioactive compounds. Students perform all the exercises practically and individually.

Students learn and practically apply the basic techniques of analysis, identification and determination of the structure of organic compounds.

That includes:

- spectroscopic methods, in particular proton nuclear magnetic resonance (1H NMR), UV-Vis and IR spectroscopy

- basic chromatographic techniques - Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC)

- techniques of separation and isolation of organic compounds: silica gel column chromatography, extraction, distillation, crystallization

- classical methods of chemical analysis

In addition, students will refere their own results to available literature data (in particular, spectral databases), thus practicing the ability to interpret the results and also practicing common ways recording data in accordance with accepted standards.

The laboratory also strengthens the previously adopted standards of work in accordance with health and safety regulations, as well as the ability to conduct observations and laboratory notes.

Particular procedures of synthesis and instructions for exercise are provided, in advance, by the instructors.

Two excused absences are allowed, although all exercises must be completed.

Due to sanitary requirements, some classes (in particular - NMR and IR spectroscopy) might be organized in a form of distance learning.

(all literature is given in Polish, for english version please contact the teacher)

1. K. Bańkowski, A. Krawczyk, R. Siciński, J. Stępiński, A. Temeriusz – Ćwiczenia z organicznej analizy jakościowej i chemii bioorganicznej - Wyd. UW, 1990.

2. A. Vogel - Preparatyka organiczna - WNT, 1984

3. Z. Witkiewicz - Podstawy chromatografii i technik elektromigracyjnych - PWN, 2017

4. Z. Witkiewicz – Podstawy chromatografii - WNT, 2000

5. M. Szafran, Z. Dega-Szafran - Określanie struktury związków organicznych metodami spektroskopowymi - PWN, 1988

6. R. M. Silverstein, F.X. Webster, D.J. Kiemle Spektroskopowe metody identyfikacji związków organicznych - PWN 2007

7. W. Zieliński, A. Rajca (red) - Metody spektroskopowe i ich zastosowanie do identyfikacji związków organicznych - WNT, 1995

8. C.N.R. Rao, – Spektroskopia elektronowa związków organicznych - PWNT, 1982

9. J. McMurry - Chemia organiczna, części 1-5 - PWN, 2007 (lub późniejsze): rozdziały dotyczące określania struktury związków

10. J. Clayden, N. Greeves, S. Warren, P. Wothers - Chemia organiczna - WNT, 2009 (lub późniejsze): rozdziały dotyczące określania struktury związków

After completing the course the student:

- can propose a rational method of separation of a mixture of compounds, isolation and purification of its components and can practically perform simple distillation, extraction, crystallization and purification using column chromatography,

- can choose the eluent system and perform thin layer chromatography (TLC),

- can perform HPLC and GC analysis, also using standards, understand the results of these analyzes in the context of assessing the purity of the samples

- can propose appropriate spectral techniques, in particular 1H NMR, UV-Vis, IR for the structural identification of organic compounds and assessment of their purity

- can experimentally perform 1H NMR, UV-Vis, IR analysis, taking into account the requirements of each technique (solvent / matrix, concentration range, sample preparation, appropriate technical equipment)

- knows the basic methods of classical chemical identification of organic compounds

- can interpret the results of published spectral and chromatographic analyzes and record the results of own analyzes in accordance with accepted standards

- knows the basic academic spectral databases and software, in particular MestreNova for 1H NMR

- Requirements for passing the course:

1) Completion of all individual excerices and approval of written reports of each excercise

2) Passing all the partial test. Each test consists of two parts - theoretical (8 points) and concerning the performance of a given exercise (2 points). It is required to obtain at least 50% from all partial tests and at least 50% from the theoretical part of each test (4 points). The theoretical parts of each test can be corrected during the semester during classes upon appointment. Students propose when they want to retake tests. The part of the test concerning the performance of a given exercise cannot be corrected.

- The final grade consists of:

1) grades obtained from tests,

2) grades obtained from exercises,

3) assessment of the overall quality of work, in particular compliance with the requirements of occupational health and safety.

- Conditions for passing in the correction session:

Students who did not complete all practical exercises within the deadline may perform ONE exercise during the correction session. Failure to perform more than one exercise makes it impossible to pass a course.

Students who did not meet the credit criteria due to tests criteria can correct any number of tests in a maximum of two appointments.

Schedules for retaking the tests and practical exercises are set individually.

Not applicable