Print syllabusApplication of Scanning Probe Microscopy to Biological Samples
General data
| Course ID: | 1200-2MON29L |
| Erasmus code / ISCED: |
13.3
|
| Course title: | Application of Scanning Probe Microscopy to Biological Samples |
| Name in Polish: | Skaningowa mikroskopia tunelowa oraz mikroskopia sił atomowych w badaniach powierzchni o charakterze biologicznym |
| Organizational unit: | Faculty of Chemistry |
| Course groups: |
(in Polish) Przedmioty do wyboru w semestrze 3M (S2-PRK-CHM) (in Polish) Wykłady monograficzne (S2-CH, S2-CHS) (in Polish) Wykłady monograficzne w semestrze letnim (S2-CH, S2-CHS) |
| ECTS credit allocation (and other scores): |
1.50
|
| Language: | Polish |
| Main fields of studies for MISMaP: | biology |
| Type of course: | elective monographs |
| Prerequisites (description): | Fundamental knowledge about intermolecular interactions and surface chemistry. |
| Mode: | Classroom |
| Short description: |
Fundamentals of scanning tunneling microscopy (STM) and atomic force microscopy (AFM) will be presented, including different imaging modes, as well as other applications beyond imaging. In particular, microscopic studies of some biological and biomimetic surfaces will be reviewed. |
| Full description: |
Fundamentals of scanning tunneling microscopy (STM) and electrochemical scanning tunneling microscopy (EC-STM). Application of STM and EC-STM techniques for structural studies of biomimetic membranes including lipid films. Application of STM for molecular recognition imaging, identification of functional groups and metallic centers within the proteins, electron transfer through organic molecules. Fundamentals of atomic force microscopy (AFM). Different AFM modes: Contact (C-AFM), Non-contact/Intermittent contact (NC/IC-AFM), Conductive Probe (CP-AFM), Lateral Force (LFM), Electrostatic Force (EFM), Chemical Force (CFM), Force Spectroscopy. Imaging and probing of biological samples, biomimetic membranes and cells. Measurements of adhesion forces on model membranes. Chemical specificity in AFM-based imaging. Modification of AFM probes with (bio)molecules for molecular recognition imaging and the identification of specific membrane regions. |
| Bibliography: |
1. "Scanning Probe Microscopies Beyond Imaging Manipulation of Molecules and Nanostructures" ed. Paolo Samori, Wiley VCH, Weinheim 2006. 2. "A practical Guide to Scanning Probe. Microscopy.", R. Howland, L. Benatar. Park Scientific Instruments, 1993-1996 3. P.W. Atkins "Physical Chemistry" Oxford Univeristy Press, 2006. 4. 4. "Review of Progress in Atomic Force Microscopy" S. Maghsoudy-Louyeh, M. Kropf, B. R. Tittmann, 2018, DOI: 10.2174/1874440001812010086. |
| Learning outcomes: |
General knowledge about • the fundamentals of STM and AFM techniques and the mechanism of contrast • how to select proper SPM technique for particular sample • application of STM and AFM beyond conventional imaging – especially for biological and biomimetic samples. |
| Assessment methods and assessment criteria: |
Assessment in the form of a quiz. The pass mark is to obtain a minimum of 51% of the maximum number of points in the quiz. The permissible number of excusable absences is 2. |
| Practical placement: |
N/A |
Classes in period "Summer semester 2023/24" (in progress)
| Time span: | 2024-02-19 - 2024-06-16 |
 
MO WYK-MON
TU W TH FR |
| Type of class: |
Monographic lecture, 15 hours, 50 places
|
|
| Coordinators: | Sławomir Sęk | |
| Group instructors: | Sławomir Sęk | |
| Students list: | (inaccessible to you) | |
| Examination: | Grading |
Copyright by University of Warsaw.
