Application of Scanning Probe Microscopy to Biological Samples

General data

Course ID:	1200-2RMON2L
Erasmus code / ISCED:	13.3 Kod klasyfikacyjny przedmiotu składa się z trzech do pięciu cyfr, przy czym trzy pierwsze oznaczają klasyfikację dziedziny wg. Listy kodów dziedzin obowiązującej w programie Socrates/Erasmus, czwarta (dotąd na ogół 0) – ewentualne uszczegółowienie informacji o dyscyplinie, piąta – stopień zaawansowania przedmiotu ustalony na podstawie roku studiów, dla którego przedmiot jest przeznaczony. / (0531) Chemistry The ISCED (International Standard Classification of Education) code has been designed by UNESCO.
Course title:	Application of Scanning Probe Microscopy to Biological Samples
Name in Polish:	Skaningowa mikroskopia tunelowa oraz mikroskopia sił atomowych w badaniach powierzchni o charakterze biologicznym
Organizational unit:	Faculty of Chemistry
Course groups:	(in Polish) Wykłady monograficzne w semestrze letnim 2M Radiogenomika (S2-PRK-RAD)
ECTS credit allocation (and other scores):	(not available) Basic information on ECTS credits allocation principles: the annual hourly workload of the student’s work required to achieve the expected learning outcomes for a given stage is 1500-1800h, corresponding to 60 ECTS; the student’s weekly hourly workload is 45 h; 1 ECTS point corresponds to 25-30 hours of student work needed to achieve the assumed learning outcomes; weekly student workload necessary to achieve the assumed learning outcomes allows to obtain 1.5 ECTS; work required to pass the course, which has been assigned 3 ECTS, constitutes 10% of the semester student load. view allocation of credits
Language:	Polish
Type of course:	elective courses
Mode:	Classroom
Short description:	Fundamentals of scanning tunneling microscopy (STM) and atomic force microscopy (AFM) will be presented, including different imaging modes, as well as other applications beyond imaging. In particular, microscopic studies of some biological and biomimetic surfaces will be reviewed.
Full description:	Fundamentals of scanning tunneling microscopy (STM) and electrochemical scanning tunneling microscopy (EC-STM). Application of STM and EC-STM techniques for structural studies of biomimetic membranes including lipid films. Application of STM for molecular recognition imaging, identification of functional groups and metallic centers within the proteins, electron transfer through organic molecules. Fundamentals of atomic force microscopy (AFM). Different AFM modes: Contact (C-AFM), Non-contact/Intermittent contact (NC/IC-AFM), Conductive Probe (CP-AFM), Lateral Force (LFM), Electrostatic Force (EFM), Chemical Force (CFM), Force Spectroscopy. Imaging and probing of biological samples, biomimetic membranes and cells. Measurements of adhesion forces on model membranes. Chemical specificity in AFM-based imaging. Modification of AFM probes with (bio)molecules for molecular recognition imaging and the identification of specific membrane regions.
Bibliography:	1. "Scanning Probe Microscopies Beyond Imaging Manipulation of Molecules and Nanostructures" ed. Paolo Samori, Wiley VCH, Weinheim 2006. 2. "A practical Guide to Scanning Probe. Microscopy.", R. Howland, L. Benatar. Park Scientific Instruments, 1993-1996 3. P.W. Atkins "Physical Chemistry" Oxford Univeristy Press, 2006. 4. 4. "Review of Progress in Atomic Force Microscopy" S. Maghsoudy-Louyeh, M. Kropf, B. R. Tittmann, 2018, DOI: 10.2174/1874440001812010086.
Learning outcomes:	General knowledge about • the fundamentals of STM and AFM techniques and the mechanism of contrast • how to select proper SPM technique for particular sample • application of STM and AFM beyond conventional imaging – especially for biological and biomimetic samples.
Assessment methods and assessment criteria:	Assessment in the form of a quiz. The pass mark is to obtain a minimum of 51% of the maximum number of points in the quiz. The permissible number of excusable absences is 2.

This course is not currently offered.

Course descriptions are protected by copyright.
Copyright by University of Warsaw.