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Liquid chromatography mass spectrometry

General data

Course ID: 1200-2SPEC332M
Erasmus code / ISCED: 13.3 Kod klasyfikacyjny przedmiotu składa się z trzech do pięciu cyfr, przy czym trzy pierwsze oznaczają klasyfikację dziedziny wg. Listy kodów dziedzin obowiązującej w programie Socrates/Erasmus, czwarta (dotąd na ogół 0) – ewentualne uszczegółowienie informacji o dyscyplinie, piąta – stopień zaawansowania przedmiotu ustalony na podstawie roku studiów, dla którego przedmiot jest przeznaczony. / (0531) Chemistry The ISCED (International Standard Classification of Education) code has been designed by UNESCO.
Course title: Liquid chromatography mass spectrometry
Name in Polish: Chromatografia cieczowa sprzężona ze spektrometrią mas
Organizational unit: Faculty of Chemistry
Course groups: (in Polish) Przedmioty do wyboru w semestrze 3M (S2-PRK-CHM)
(in Polish) Wykłady specjalizacyjne w semestrze 2M
ECTS credit allocation (and other scores): 3.00 Basic information on ECTS credits allocation principles:
  • the annual hourly workload of the student’s work required to achieve the expected learning outcomes for a given stage is 1500-1800h, corresponding to 60 ECTS;
  • the student’s weekly hourly workload is 45 h;
  • 1 ECTS point corresponds to 25-30 hours of student work needed to achieve the assumed learning outcomes;
  • weekly student workload necessary to achieve the assumed learning outcomes allows to obtain 1.5 ECTS;
  • work required to pass the course, which has been assigned 3 ECTS, constitutes 10% of the semester student load.
Language: Polish
Type of course:

obligatory courses
Prerequisites (description):

Analytical and physical chemistry for undergraduate students, basic knowledge of liquid chromatography
Mode:

Blended learning
Short description:

Course on liquid chromatography hyphenated with mass spectrometry is offered aimed to broaden understanding the idea of ths analytical technique. The fundametal aspects of mass spectrometry are described containing different analysers, ionization sources, fragmentation spectra, typical modes in MS, practical applications od LC/MS.

The course covers the stationary classes and e-learning courses, in which students solve tests and homework.

Lessons are in the first part of semester.
Full description:

The lecture comprises the following topics:

1. Fundamentals of mass spectrometry

2. Analysers in Mass spectrometry (quadrupole, ion trap, time of flight etc)

3. Ionization modes in MS

4. Tandem mass spectrometry

5. Scans modes in MS (single ion monitoring, product ion scan, single reaction monitoring, netral loss precursor ion scan etc)

6. Interpretation of mass spectrum
Bibliography:

1.R.A.W. Johnstone, M.E. Rose, Mass spectrometru for chemists and biochemists,Cambridge University Press 1996

2. E. de Hoffmann, J. Charette, V.Stroobant.Mass spectrometry principles and application ,John Wiley & Sons, Ltd 2002.

3. R. E. Ardrey, Liquid Chromatographu mass spectrometry, John Wiley & Sons, Ltd 2003
Learning outcomes:

Student knows and understands the principles of LC/MS and how to use it in practical aspects. Student knows how to interprate the simple mass spectrum. Student knows how to choose the method of ionization and how to prepare samples for LC/MS analysis.
Assessment methods and assessment criteria:

Performing all housework. Written test
Practical placement:

not

Classes in period "Summer semester 2023/24" (in progress)

Time span: 2024-02-19 - 2024-06-16
Selected timetable range:
Navigate to timetable
Type of class:
Specialised lecture, 30 hours, 50 places more information
Coordinators: Magdalena Biesaga
Group instructors: Magdalena Biesaga
Students list: (inaccessible to you)
Examination: Examination
Course descriptions are protected by copyright.
Copyright by University of Warsaw.
Krakowskie Przedmieście 26/28
00-927 Warszawa tel: +48 22 55 20 000 https://uw.edu.pl/ contact accessibility statement USOSweb 7.0.3.0 (2024-03-22)