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Liquid chromatography mass spectrometry

General data

Course ID:	1200-SPORSchcs
Erasmus code / ISCED:	13.3 Kod klasyfikacyjny przedmiotu składa się z trzech do pięciu cyfr, przy czym trzy pierwsze oznaczają klasyfikację dziedziny wg. Listy kodów dziedzin obowiązującej w programie Socrates/Erasmus, czwarta (dotąd na ogół 0) – ewentualne uszczegółowienie informacji o dyscyplinie, piąta – stopień zaawansowania przedmiotu ustalony na podstawie roku studiów, dla którego przedmiot jest przeznaczony. / (0531) Chemistry The ISCED (International Standard Classification of Education) code has been designed by UNESCO.
Course title:	Liquid chromatography mass spectrometry
Name in Polish:	Chromatografia cieczowa sprzężona ze spektrometrią mas
Organizational unit:	Faculty of Chemistry
Course groups:
ECTS credit allocation (and other scores):	3.00 Basic information on ECTS credits allocation principles: the annual hourly workload of the student’s work required to achieve the expected learning outcomes for a given stage is 1500-1800h, corresponding to 60 ECTS; the student’s weekly hourly workload is 45 h; 1 ECTS point corresponds to 25-30 hours of student work needed to achieve the assumed learning outcomes; weekly student workload necessary to achieve the assumed learning outcomes allows to obtain 1.5 ECTS; work required to pass the course, which has been assigned 3 ECTS, constitutes 10% of the semester student load.
Language:	Polish
Type of course:	obligatory courses
Short description:	The lecture aims to familiarize the student with the advanced problems of high-performance liquid chromatography coupled with mass spectrometry. The following will be discussed: the construction of the apparatus, the modes of operation of the mass spectrometer, and the types of ionization in LC-MS
Full description:	The lecture covers the following topics: 1. Basics of mass spectrometry, 2. Analyzers in mass spectrometry, 3. Methods of ionization of compounds in LC-MS, 4. Tandem mass spectrometry, 5. Modes of work of mass spectrometers (monitoring of a selected ion, monitoring of fragmentation ions, loss of a neutral molecule, monitoring of selected fragmentation reactions, etc.). The lecture will be supplemented with two laboratory exercises: 1. Determination of polyphenolic compounds by LC/MS method, 2. Identification of natural dyes in antique fabrics.
Bibliography:	(in Polish) 1.R.A.W. Johnstone, M.E. Rose, Spektrometria mas, PWN, 2001 2. E. de Hoffmann, J. Charette, V. Stroobant, Spektrometria mas, Wydawnictwo Naukowo-Techniczne, Warszawa, 1994 3. W. Danikiewicz, Spektrometria mas, Warszawa PWN 2020
Learning outcomes:	(in Polish) Student wie i rozumie na czym polega proces rozdzielania związków przy zastosowaniu LC/MS. Student potrafi zinterpretować proste widmo MS i potrafi dobrać warunki detekcji ms do analizy związków organicznych. Student wie jak należy przygotować próbkę do analizy z wykorzystaniem LC/MS.
Assessment methods and assessment criteria:	(in Polish) Egzamin końcowy przeprowadzany w formie egzaminu pisemnego, pytania otwarte. Warunkiem zaliczenia jest uzyskanie powyżej 50% punktów.

Classes in period "Academic year 2023/24" (in progress)

Time span:	2023-10-01 - 2024-06-16	Selected timetable range: weekly course term Navigate to timetable
Type of class:	Lab, 10 hours more information Lecture, 2 hours more information
Coordinators:	Magdalena Biesaga, Tomasz Gierczak
Group instructors:	Magdalena Biesaga
Students list:	(inaccessible to you)
Examination:	Grading

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