(in Polish) Monitoring organizmów genetycznie zmodyfikowanych

general courses

Cartagena Protocol ; Directive 2001/18/EC of the European Parlament and of the Council; The main objectives of GMOs monitoring; Relationships with environmental risk assessment and testing of analytical hypothesis; Criteria for selecting the time scale, spatial (area), and objects for monitoring; Monitoring of GMO in environment and in food; Search for transgenes in wild-type plants; Case Specific Monitoring - objectives, approach, substantive and experimental methodology; Overview of the methods, their advantages and disadvantages, sensitivity, adjust the method for the purpose of monitoring, the order of the proceedings; Examples of most frequently monitored crops and microorganisms;

LECTURE

Definition of genetically modified organisms and the methods used to produce GMOs; the Cartagena Protocol on Biosafety to the

Convention on Biological Diversity, Directive 2001/18/EC of the European Parliament and of the Council and their references to the Convention on Biological Diversity; GM bacteria monitoring objectives; The objectives of the monitoring of GM crops; Monitoring as a tool of environmental risk assessment; Case Specific Monitoring - short-and long-term monitoring the effects of the release into the environment of selected genetically modified plants and bacteria, adjusting the spatial scale for the purpose of monitoring, assessment of environmental risks; Monitoring of GMOs in processed foods, fresh, crops in the fields; Detection techniques of GM microorganism based on DNA analysis; The most commonly used vectors, marker genes, genes modifying the characteristics of microorganisms, GM microorganisms and horizontal gene transfer; Methods for determining the number of GM microorganisms (enzymatic, fluorescent, PCR, MPN-PCR, real-time PCR); General Surveillance - monitoring as a tool to control harmful for human health effects of the use of GM crops; Monitoring the presence of GM plants, parts of their bodies and products in the environment; Range of monitoring of GM crops: all organisms in the environment (crops and wild type after crossing from GMOs), parts of the body (including pollen, fragments of bodies) in the air, water, soil, sewage sludge, food fresh and processed in the gastrointestinal tract of animals); Identifying ways of spread GM crops in the environment; The rules for creating a plan for monitoring progress (including the response to the demand, the choice of indicators, methods, places, method of preparation of the report); The most commonly used vectors, marker genes, genes modifying the characteristics of plants; The most common species of plants and modified features; Methods of monitoring, taking into account the duration and site selection (including types of methods, sensitivity, adaptation to needs), standardization of methods; Principles registration of GM crops.

Having completed the course and the lab the student:

KNOWLEDGE

-has the knowledge about modern methods and techniques used in the evaluation of the natural environment

- knows and understands the terminology and basic methods (research facilities and measurement) of environmental monitoring,

ABILITIES

- knows how to independently plan and perform research tasks and expertise

- has the ability to collect empirical data and their interpretation.

SOCIAL AWARENESS

- has a habit of using objective scientific information sources

Having completed the course and the lab the student:

KNOWLEDGE

-has the knowledge about modern methods and techniques used in the evaluation of the natural environment

- knows and understands the terminology and basic methods (research facilities and measurement) of environmental monitoring,

ABILITIES

- knows how to independently plan and perform research tasks and expertise

- has the ability to collect empirical data and their interpretation.

SOCIAL AWARENESS

- has a habit of using objective scientific information sources

Lectures - Attendance at the lecture is monitored by signing the attendance list; obligatory participation (three absences possible)

Assessment is based on the test exam, which deals with all matters mentioned during lectures and class (60% of correct answers is needed to pass the exam).