Bioprocessing Engineering

obligatory courses

The lecture focuses on principles of industrial processes involving microorganisms, enzymes, plant and animal cells. The bioreactor engineering, down- and up-stream processing are the main subjects presented.

Bioreactor engineering includes: kinetics of enzyme reactions, elementary balances of microbial growth, models of microbial growth, submerged cultures and solid state fermentation, immobilization of enzymes and cells, mass and heat transfer in bioreactors and scale-up of bioreactors.

Up-stream processing includes: sterilization, media optimization and methods of solid substances milling and mixing.. Down-stream processing includes: biomass separation, cell disruption, precipitation, membrane methods of separation, chromatography, extraction, distillation and rectification and drying of biological materials

The lecture focuses on principles of industrial processes involving microorganisms, enzymes, plant and anima cells. The bioreactor engineering, down- and up-stream processing are the main subject presented.

1.Bioreactor engineering principles 16 h

1.1.Balances of microbial growth 4 h

Elemental balances, degree of reduction, energetic of growth, maintenance, structural balances.

1.2.Enzymatic kinetics 2 h

Michaelis - Menten kinetics. Estimation of .Michalis-Menten model coefficients. Mechanisms of inhibition.. Effects of temperature and pH.. Complex enzymatic reactions

1.3.Microbial growth models 1 h

Model of microbial kinetics. Non-structured and structured models. Segregated models.

1.4.Reactor engineering principles. 1 h

Ideal and non-ideal reactors. Macro and micro-mixing.

1.5.Submerged cultures. 2 h

Batch culture. Fed-batch cultures. Continuous cultures.

1.6.Solid state fermentation. 1 h

1.7.Enzymatic reactors. 1 h

1.8.Bioreactors with immobilized enzymes and cells. 1 h

1.9.Membrane bioreactors. 1 h

1.10.Design of bioreactors. 2 h

Aeration and mixing systems. Heat transfer in bioreactors. Scale-up of bioreactors.

2.Up-stream processing 3 h

2.1.Optimizing of medium compositions 1 h

2.2.Sterilization. 1 h

Sterilization techniques. Thermal sterilization. Absolute filtration.

2.3.Disruption and mixing of solid materials. 1 h

3.Down-stream processing. 11 h

3.1.Biomass separation 1 h

Filtration. Centrifugation. Sedimentation

3.2.Cell disruption.. 1 h

3.3.Membrane methods of separation. 2 h

Dialysis. Elektrodialysis.. Ultrafiltration. Reverse osmosis.

3.4.Electrokinetic and chromatography methods. 1 h

3.5.Precipitation. 1 h

3.6.Extraction 1 h

3.8.Distillation and rectification 2 h.

3.9.Drying of biological materials. 1 h

3.10.Selection of separation methods. 1 h

1. P.Doran, Bioprocess Engineering Principles, Academic Press, London, 1995

2. M.L.Shuler, F.Kargi, Bioprocess Engineering. Basic Concepts, Prentice Hall PTR, New Jersey, 2002

3. H.W. Blanch, D.S. Clark, Biochemical Engineering, Marcel Dekker Inc., New York, 1996