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Proteomics

General data

Course ID:	1400-225PROT-en
Erasmus code / ISCED:	13.104 Kod klasyfikacyjny przedmiotu składa się z trzech do pięciu cyfr, przy czym trzy pierwsze oznaczają klasyfikację dziedziny wg. Listy kodów dziedzin obowiązującej w programie Socrates/Erasmus, czwarta (dotąd na ogół 0) – ewentualne uszczegółowienie informacji o dyscyplinie, piąta – stopień zaawansowania przedmiotu ustalony na podstawie roku studiów, dla którego przedmiot jest przeznaczony. / (0511) Biology The ISCED (International Standard Classification of Education) code has been designed by UNESCO.
Course title:	Proteomics
Name in Polish:	Proteomics
Organizational unit:	Faculty of Biology
Course groups:	(in Polish) Przedmioty obieralne na studiach drugiego stopnia na kierunku bioinformatyka Elective subjects Facultative courses, BIOLOGY, specialization level (2nd study cycle), spec.: BM Requisite, complementary subjects, BIOTECHNOLOGY, 2nd study cycle, specialization: BM Specific programme courses of 2nd stage Bioinformatics
ECTS credit allocation (and other scores):	9.00 Basic information on ECTS credits allocation principles: the annual hourly workload of the student’s work required to achieve the expected learning outcomes for a given stage is 1500-1800h, corresponding to 60 ECTS; the student’s weekly hourly workload is 45 h; 1 ECTS point corresponds to 25-30 hours of student work needed to achieve the assumed learning outcomes; weekly student workload necessary to achieve the assumed learning outcomes allows to obtain 1.5 ECTS; work required to pass the course, which has been assigned 3 ECTS, constitutes 10% of the semester student load.
Language:	English
Type of course:	optional courses
Mode:	Classroom
Short description:	In this course students get acquainted with the proteomic methodology
Full description:	In this course students get acquainted with the proteomic methodology. In practical part students will purify protein complexes by Tandem Affinity Purification method and analyze there composition using Mass Spectrometry. In theoretical part students will gain knowledge about various applications of Mass Spectrometry in biology and medicine. This includes the application of mass spectrometry for the identification of proteins and their posttranslational modifications, the information on typical protocols of sample fractionation and preparation, types of mass spectrometric experiments used (including data dependent MS/MS experiment as a basis of modern proteomic analysis) and software tools for the data analysis (database search) and verification steps. Lecture is illustrated with the examples of application of proteomic methodology for solving various problems of bio-medical sciences.
Bibliography:	Gary Siuzdak (1996) Mass Spectrometry for Biotechnology, Academic Press (ISBN 0-12-647471-0) - brak polskiej pozycji dot. proteomiki. E. de Hoffmann, J. Chatette, V. Stroobant (1994) Spectrométrie de masse; Masson Editeur, Paris; tłum. L. Konopski (1998) Spektrometria Mas; Wydawnictwa Naukowo-Techniczne (ISBN 83-204-2291-4). Robert A. W. Johnstone, Malcolm E. Rosse (1996) Mass spectrometry for chemists and biochemists; Press Syndicate of the University of Cambridge; tłum. pod red. Krystyny Jurkowskiej (2001) Spektrometria mas; Wydawnictwo Naukowe PWN SA (ISBN 83-01-13605-7).
Assessment methods and assessment criteria:	The final assessment is based on following components: 1. assessment of activity in class. 2. mid-semester presentation on the proteins purified and progress of the experiment. 3. final report and presentation at the end of class.

Classes in period "Winter semester 2023/24" (past)

Time span:	2023-10-01 - 2024-01-28	Selected timetable range: weekly course term Navigate to timetable MO TU LAB W TH FR
Type of class:	Lab, 90 hours more information
Coordinators:	Michał Dadlez, Andrzej Dziembowski, Seweryn Mroczek
Group instructors:	Andrzej Dziembowski, Seweryn Mroczek
Students list:	(inaccessible to you)
Examination:	Course - Examination Lab - Examination
Notes:	(in Polish) Warunki przjęcia na zajęcia Przyjmowani będą studenci pierwszego roku studiów magisterskich. Informację o ocenie/ocenach i/lub preferencjach grup zajęciowych należy wpisać do załączonego formularza: https://docs.google.com/forms/d/e/1FAIpQLSckt9Hau6mM9xY81k5TZCHFISFDtTM-EeWrmR0oB2VdpjMTIQ/viewform?usp=sharing

Classes in period "Winter semester 2024/25" (future)

Time span:	2024-10-01 - 2025-01-26	Selected timetable range: weekly course term Navigate to timetable MO TU LAB W TH FR
Type of class:	Lab, 90 hours more information
Coordinators:	Michał Dadlez, Andrzej Dziembowski, Seweryn Mroczek
Group instructors:	Andrzej Dziembowski, Seweryn Mroczek
Students list:	(inaccessible to you)
Examination:	Course - Examination Lab - Examination
Notes:	(in Polish) Warunki przjęcia na zajęcia Przyjmowani będą studenci pierwszego roku studiów magisterskich. Informację o ocenie/ocenach i/lub preferencjach grup zajęciowych należy wpisać do załączonego formularza: https://docs.google.com/forms/d/e/1FAIpQLSckt9Hau6mM9xY81k5TZCHFISFDtTM-EeWrmR0oB2VdpjMTIQ/viewform?usp=sharing

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