Cytometry - applications in biology research

elective courses

Basic knowledge in cellular and molecular biology

Classroom

1. Overview of flow and image cytometry instrumentation.

2. Safety procedures and quality control.

3. Handling, storage and preparation of molecular and cellular probes.

4. Overview of cytometric methods: phenotypic analysis, nucleic acid analysis, cell function studies, clinical applications.

5. Data processing and analysis.

6. Data presentation

list of topics:

1. Introduction to cytometry - how it works.

2. Characteristic of antibodies, fluorochromes and fluorescent proteins.

3. Flow and image cytometry instrumentation.

4. Preparation of samples for cytometric analysis.

5. Analysis of expression of surface and intracellular markers.

6. Analysis of cell cycle cell, apoptosis and necrosis.

7. Other application of flow cytometry in biological study.

8. Clinical application of flow cytometry.

9. Application of image cytometry

10. Cell sorting.

11. Data processing and advanced multiplex analysis.

12. Data presentation.

1. Practical Flow cytometry H. M. Shapiro, 2003 Wiley-liss

2. Current protocols in cytometry, Wiley

Knowledge

1. Is familiar with theoretical basis of flow cytometer functioning and samples designing. (K_W12)

2. Knows basic differences in various types cytometers and sorters operation and softwares. (K_W10)

3. Has broad knowledge in application of flow cytometry for phenotypical and functional analysis of immune cells. (K_W13)

4. Has knowledge in different application of flow cytometry for analysis of prokaryotic and eukaryotic cells. (K_W10)

Skills

1 .Ability to use cytometers FACSCalibur and Verse, sorter FACSAria , High Content Imaging device Pathway. (K_U01)

2 .Ability to prepare cells for flow cytometric acquisition and analysis.(K_U01)

3. Ability to use CellQuest, Diva and FACSuite software for cell analysis. (K_U01)

4. Is able to plan experiments, draw proper conclusions and interpretation of research results based on the obtained data under control of supervisor. (K_U07)

Social skills

1. Is responsible for the organization of safety conditions for conducted experiments and is able to evaluate the level of hazard resulting from experimental techniques. (K_K08)

2. Is responsible for the research which has been assigned to him/her, for his/her own laboratory work and other people’s work. (K_K04)

Written exam with open questions.

Not required.